Journal: The Journal of Biological Chemistry

Article Title: O-GlcNAc transferase couples nutrient availability to synaptic plasticity in paraventricular neurons to regulate satiety

doi: 10.1016/j.jbc.2025.111124

Figure Lengend Snippet: Nutritional state differentially modulates synaptic transmission in WT and OGT-KO neurons. A , representative trace showing suppression of spontaneous excitatory synaptic events following NBQX application in WT PVN αCaMKII neurons. The red arrow represents a typical excitatory spike. B – D , representative Western blots and quantification of total and surface GluA1 expression in primary cortical neurons. WT values were normalized to 1. OGT-KO total GluA1 = 1.282 ± 0.068; surface GluA1 = 1.629 ± 0.064. E–G , representative Western blots and quantification of total and surface GluA2 expression. WT values were normalized to 1. OGT-KO total GluA2 = 0.7101 ± 0.081; surface GluA2 = 0.5138 ± 0.075. H–J , representative Western blots and quantification of total and surface GluA3 expression. WT values were normalized to 1. OGT-KO total GluA3 = 0.6691 ± 0.032; surface GluA3 = 0.3935 ± 0.044. Western blot quantitative analyses were performed using the Wilcoxon signed-rank test. All Western blot data expressed as mean ± SEM. K , representative sEPSC traces in WT neurons under hungry ( top ) and fed ( bottom ) conditions. L , sEPSC frequency in WT neurons comparing hungry vs fed states ( p = 0.021). M , sEPSC amplitude in WT neurons comparing hungry vs fed states (NS, p = 0.851). N , sEPSC decay tau in WT neurons comparing hungry vs fed states (NS, p = 0.142). O , Representative sEPSC traces in OGT-KO neurons under hungry ( top ) and fed ( bottom ) conditions. P , sEPSC frequency in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.095). Q , sEPSC amplitude in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.309). R , sEPSC decay tau in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.547). All sEPSC data ( panels K–R ) were obtained from WT (n = 5) and OGT-KO (n = 5) mice under hungry and fed conditions (n = 5 each condition). Statistical comparisons using Mann-Whitney non-parametric test. Data presented as mean ± SEM. Blue bars: WT neurons ( dark blue = hungry, light blue = fed); yellow bars: OGT-KO neurons ( light yellow = hungry, bright yellow = fed). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, NS, non-significant.

Article Snippet: Membranes were blocked in 5% non-fat milk prepared in TBS-T and incubated overnight at 4 °C with primary antibodies against OGT (Proteintech, 11576-2-AP; 1:2000), HSP70 (Proteintech, 10995-1-AP; 1:10,000), GluA1 (NeuroMab, N355/1; 5μg/blot), GluA2 (NeuroMab, L21/32; 5μg/blot), or GluA3 (Almone labs, AGC-010-GP; 1:1000).

Techniques: Transmission Assay, Western Blot, Expressing, MANN-WHITNEY

Journal: npj Imaging

Article Title: Quantitative multi-metabolite imaging of Parkinson’s disease using AI boosted molecular MRI

doi: 10.1038/s44303-025-00130-x

Figure Lengend Snippet: The white dashed lines in all panels mark the cortex-striatum boundary. a Glutamate (Glu) and DAPI staining. The white arrows highlight Glu-positive signals in the striatum, presenting an increase after MPTP administration. b Glu receptor (mGluR3) and DAPI staining. c Glu and mGluR3 receptor staining. The yellow arrows indicate double-stained areas in the striatum, demonstrating enhanced expression in the MPTP-treated mouse. d GFAP and DAPI staining, with an increased GFAP signal in the MPTP group, reflecting higher astrocytic activation (with DAPI providing background cell body visualization). e A merged image combining the signals from ( a , b , d ). f Coomassie blue staining. The increased staining intensity in the MPTP-treated brain suggests an increase in total protein content.

Article Snippet: Antibodies against glutamate (#AB5018, Sigma Aldrich), GFAP (#Ab4674, Abcam), and mGluR3 glutamate receptor (#AGC-010-GP, Alomone labs) were used for IHC tissue staining.

Techniques: Staining, Expressing, Activation Assay

Journal: iScience

Article Title: GluK2 Q/R editing regulates kainate receptor signaling and long-term potentiation of AMPA receptors

doi: 10.1016/j.isci.2023.107708

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal Anti-GluA3 , Alomone , RRID: AB_2039883.

Techniques: Recombinant, Protein Extraction, Software

Journal: Pharmaceuticals

Article Title: Anti-AMPA Receptor Autoantibodies Reduce Excitatory Currents in Rat Hippocampal Neurons

doi: 10.3390/ph16010077

Figure Lengend Snippet: Rabbit anti-AMPAR GluR3B immunogenicity response. ( A ) ELISA of protein A-purified AMPAR (GluR3) Aabs against the GluR3 immunisation peptide or an irrelevant peptide; n = 3 technical replicates. ( B ) Western blot of mouse whole brain lysate probed with a commercial anti-AMPAR antibody (cAMPAR), anti-AMPAR Aabs, a class-specific negative control rIgG (naïve) or secondary antibody only (negative control). Representative blots from n = 3 technical replicates. ( C ) Immunocytochemical staining of fixed primary cortical neuron on cultures. Cells (DIV8) were stained with anti-AMPAR Aabs (red), anti-βIII tubulin (green), GFAP (white) and nuclei counterstained with DAPI (blue). Examples of labelling of hippocampal neurons with anti-AMPAR Aabs (red) is indicated by the white arrows. Scale bars = 20 μm. Representative images from n = 3 technical replicates.

Article Snippet: Primary and secondary antibodies used were as follows: rabbit anti-AMPAR (1:100; raised against residues 60–73 of rat GluR3 ATD, AGC-010, Alomone Labs, Jerusalem, Israel); rabbit anti-IgG 1 (rIgG, 1:100, 011-000-003, Jackson ImmunoResearch, Cambridge, UK); mouse anti-IgG 2b (1:100, 70–4732, BioLegend, London, UK); mouse anti-βIII-tubulin (mIgG2b, 1:500, 801201, BioLegend, London, UK); mouse anti-glial fibrillary acidic protein (GFAP) (1:400, MAB3402, Millipore); goat anti-rabbit or anti-mouse Alexa Fluor 488/594/647 (all at 1:1000, Life Technologies, Loughborough, UK).

Techniques: Enzyme-linked Immunosorbent Assay, Purification, Western Blot, Negative Control, Staining